|Medical/Graduate School||Iowa State University, Ames, IA|
|Clinical/Post-doctoral Fellowship||Harvard Medical School, Boston, MA|
|Research Interest||1. Development of Molecular Imaging Technology for Visualizing HIV-1 Reservoirs in Vivo
We engineer lentivirus including human, simian and feline immunodeficiency virus (HIV, SIV and FIV) as "Trojan Horse" to carry molecular imaging reporter to investigate where the virus goes and when it infect the target cells using in vivo imaging technologies based on optical bioluminescence imaging (BLI), MRI and PET.
2. Visualizing therapeutic nanoparticles for HIV-1 Eradication.
We use a novel gene editing technique called CRISPR/Cas9, which effectively targets the HIV-1 genome using small guidance RNAs (gRNAs), for eradicating HIV-1 in a preclinical setting. Non-invasive in vivo imaging methods are used to visualize the biodistribution and the effectiveness of therapeutic nanoparticles to excise HIV-1 genome from its host cells as an innovative eradication strategy.
3. Visualizing immune cells and HIV-1 infection
We visualize the interaction between immune cells and HIV-1 mucosal transmission, e.g. intravaginal transmission, and, drug-resistant HIV-1 mutants and Pre-Exposure Prophylaxis (PrEP) using 19F MRI and BLI.
|Selected Publications||Yin C, Zhang T, Li F, Yang F, Putatunda R, Young WB, Khalili K, Hu W, Zhang Y. Functional screening of guide RNAs targeting the regulatory and structural HIV-1 viral genome for a cure of AIDS. AIDS. 2016 Mar 16. [Epub ahead of print] PubMed PMID: 26990633.
Zhang Y, Yin C, Zhang T, Li F, Yang W, Kaminski R, Fagan PR, Putatunda R, Young WB, Khalili K and Hu W. CRISPR/gRNA-directed synergistic activation mediator (SAM) induces specific, persistent and robust reactivation of the HIV-1 latent reservoir. Scientific Reports, 2015, Sci. Rep. 5, 16277; doi: 10.1038/srep16277.
Song, J., Cai, Z., White, AG., Jin, T., Wang, X., Kadayakkara, D., Anderson, CJ., Ambrose, Z., Young WB. Visualization and quantification of simian immunodeficiency virus (SIV)-infected cells using non-invasive molecular imaging. J of Gen. Virology, 2015, Jul 14. doi: 10.1099/jgv.0.000245. [Epub ahead of print]. PMID: 26297664.
Zhang L., Jiang W., Young WB. Gut associated lymphoid tissue regulated intestinal barrier in HIV infection. Immuno-Gastroenterology 2013; 2:156-161.
Song J. and Young WB. Imaging lentiviral reservoirs in vivo based on molecular imaging reporter genes. J of NeuroVirology 2013 Oct; 19 (Supplement 1):S77-78.
Koukuntla R, Ramsey WJ, Young WB, Link CJ.U6 promoter-enhanced GlnUAG suppressor tRNA has higher suppression efficacy and can be stably expressed in 293 cells. J Gene Med. 2013 Feb;15(2):93-101. PMID: 23303531
Kadayakkara DK, Ranganathan S., Young WB, Ahrens ET. Assaying macrophage activity in a murine model of inflammatory bowel disease using fluorine-19 MRI. Laboratory Investigation 2012 Apr;92(4):636-45. PMID: 22330343. PMCID: PMC3397682.
Ahrens ET, Young WB, Xu H, Pusateri LK. Rapid quantification of inflammation in tissue samples using perfluorocarbon emulsion and fluorine-19 nuclear magnetic resonance. Biotechniques. 2011 Apr;50(4):229-34.
Kadayakkara DK, Janjic JM, Pusateri LK, Young WB, Ahrens ET. In vivo observation of intracellular oximetry in perfluorocarbon-labeled glioma cells and chemotherapeutic response in the CNS using fluorine-19 MRI. Magn Reson Med. 2010 Nov;64(5):1252-9.
Link CJ, Jr., Seregina T, Edleman M, Young WB, Burt RK, Kisselev S. Transduction of hematopoietic stem cells with a retroviral vector expressing the neomycin phosphotransferase gene. Bone Marrow Transplant. 2003 Aug; 32 Suppl 1:S33-5. PMID 12931238.
Young WB, Beecham EJ, Lindberg GL, Link CJ Jr. Restriction mapping of retroviral vector mutation by analyzing episomal DNA. BioTechniques 2000; 28: 562-5. PMID 10723571.
Young WB, Lindberg GL, Link CJ, Jr. DNA methylation of helper virus increases genetic instability of retroviral vector producer cells. J. Virology 2000; 74: 3177-87. PMID: 10708434. PMCID: PMC111818.
Young WB, Link CJ, Jr. Chimeric retroviral helper virus and piconavirus IRES sequence to eliminate DNA methylation for improved packaging cells. J. Virology 2000; 74: 5242-9. PMID: 10799600. PMCID: PMC110878.
Wang S, Si D, Young WB, Jacobson C, Link CJ, Jr. A novel herpes amplicon system for in vivo gene delivery. Gene Therapy 1997; 4: 1132-41. PMID 9425435
|Gamma Sigma Delta
|PubMed Publications||See a listing of publications on PubMed, a service of the National Library of medicine.|